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Fully sequenced samples of complimentary DNA synthesized from single-stranded, mammalian RNA. Various gene inserts sourced from humans, mice, monkeys, and other species are available. Ideal for use in gene cloning, as gene probes, and in cDNA library creation.
Zyagen Mouse Universal Reference cDNA is a full length first strand cDNA synthesized from Zyagen universal reference RNA which is isolated from wide variety of male and female tissues. The cDNA is primed with oligo dT and can be used in gene cloning, target sequencing and as a control for gene expression analysis by regular PCR and real time PCR. Each cDNA product is enough for 40 PCR reactions. Features: Synthesized from high quality intact universal reference total RNA. Consistent control for standard data set comparisons. Prepared from several major tissues for broad gene coverage. Made at industrial scales to minimize lot-to-lot variation. Quality Control: The integrity of each RNA sample used for cDNA synthesis is verified by denatured agarose gel electrophoresis. The purity of RNA is assessed by NanoDrop (A260/A280: 1.9-2.1). Residual DNA contamination is tested by PCR. The synthesized cDNA is also used as template PCR amplification of ß-actin gene.
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The immunoglobulin-like transcript (ILT) comprise a family of activating and inhibitory type immunoreceptors whose genes are located in the same locus that encodes killer cell Ig-like receptors (KIR) ILT4 also known as LIR-2 and LILRB2 is a type I transmembrane protein expressed primarily on monocytes and dendritic cells (DC) LILRB2 is a receptor for class I MHC antigens Recognizes a broad spectrum of HLA-A HLA-B HLA-C HLA-G and HLA-F alleles
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This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Alternatively spliced transcript variants encoding different isoforms have been described.
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